Biosensors

Development of biosensors for detection of nerve agents, organophosphorus pesticides and other highly toxic compounds of military interest.

Recognition layer consists of enzymes (cholinesterases, dehalogenases, phosphotriesterase).

The aim is to develop sensitive, selective and fast biosensors.

Antidotes

Development of new antidotes for prophylaxis and treatment of nerve agent and organophosphorus pesticide intoxications using technology of artificial neural networks and molecular modeling for prediction.

In vitro and in vivo testing of prophylactic (cholinesterase inhibitors, bioscavengers)  and therapeutic (oxime cholinesterase reactivators, bioscavengers) antidotes.

Optimization of biological properties of bioscavengers based on enzyme phosphotriesterase, preparation of enzyme conjugates with low immunogenicity.

Development of multi-compartment autoinjector delivery system for antidotes.

Preparation of new cholinesterases modulators

Synthesis, in vitro and in vivo testing of potential drugs for treatment of Alzheimer's disease and myasthenia gravis.

Newly prepared compounds are derived from tacrine, 7-methoxytacrine (7-MEOTA) and 1,1'-oxybis(methylene) bis 4-(1,1-dimethylethyl) pyridinium dichloride (SAD-128).

Oxidative stress determination

Measurement of level of selected oxidative stress-markers in biological samples.

Development of methods for measurement of antioxidants.

Analysis

Technologies for the qualitative and quantitative analyses of antidotes and chemical warfare agents in biological samples using different HPLC techniques.

Evaluation of ability of different compounds to cross animal and human blood brain barrier (BBB).

Development of decontamination means

Preparation of novel detergents which should be used as active ingredients of the solutions for biological and chemical warfare agent decontamination derived from benzalkonium salts.

Prepared compounds are tested for their disinfection and decontamination efficacy.