Differential proteome analysis of bacterial Francisella tularensis glyco- and phospho- proteins
Hernychová, L., Klimentová, J., Bílková, Z., Tichý, A., Balonová, L., Stulík, J.
Supported by the Ministry of Education, Youth and Sports, 2008–2010 (Project No.: ME08105)
Post-translational modifications (PTM) of proteins play crucial role in the assembly, degradation, structure, and function of exprimed genes. PTM of proteins of bacterial pathogens strongly influence the nature of interaction with host cell system. However, little is known about the character and function of such modifications for intracellular bacteria. The main objective of the project is focused on the analysis of Francisella tularensis proteins, their post-translational modifications as glycosylation and phoshorylation. The aim of the research is to describe their structure in context with pathogenity and virulency of various subtypes of bacterial strains, differing in level of virulency. The strategy approach will be based on technologies that combine modern separation methods with mass spectrometry structure identification. The biofunctionalized magnetic nanoparticles with lectins, metal ions or specific mono(poly)clonal antibodies permit sufficient selectivity for glyco-sylated or phoshporylate.
INDIKÁTORY II – Reverse detection of received ionizing radiation dose by monitoring of cell population changes using biophysical methods
Šinkorová, Z., Tóthová, I., Pejchal, J., Zárybnická, L., Kročová, Z., Österreicher, J.
Supported by the Czech Republic Ministry of Defence, 2008–2011 (Project No.: OVUOFVZ200809)
To establish extrapolated calibration curve for determination the received dose in individuals exposed to ionizing radiation which could be practical used in the Czech army and to design the best enlistment of this biophysical method into the therapeutic-transfer system of the Czech army.
New biological methods of the received dose determination
Šinkorová, Z., Tichý, A., Vilasová, Z., Zárybnická, L., Matiasovic, J., Faldyna, M.
Supported by the Ministry of Education, Youth and Sports, 2008–2010 (Project No.: 2B08028)
In case of accidental irradiation of a human body (irradiation accidents, inappropriate manipulation with radioactive waste, terroristic attack) the medical personnel have to cope with the fundamental task to determine the received dose of ionizing radiation in the most accurate and rapid way. Based on such analysis an appropriate treatment of irradiation disease must begin immediately. Current biodosimetric methods are time-consuming and thus do not fulfill the main task – to begin the treatment as soon as possible, preferably within 24 hr after exposure. In the attempt to provide a faster tool for an irradiation-associated body damage estimate we propose to use multiparametric flow cytometry and microarray analysis of blood lymphocytes cultivated for a defined time interval upon irradiation, which would allow for the received dose estimate by determination of apoptosis progression or changes in protein expression in lymphocyte subsets with different radiosensitivity.
NK cells and their response to ionizing irradiation
Zárybnická, L., Šinkorová, Z.
Supported by the Czech Republic Ministry of Education, Youth and Sports, 2010–2011 (Specific research project).
This study is aimed at specific radiosensitivity of NK cells to ionizing irradiation. NK cells belong to functional white blood cells which care for anti-tumour and anti-viral control in organism. Nevertheless their responces to irradiation is not clearly known yet. Their in vivo and ex vivo radiosensitivities are studied as relative representation changes in peripheral blood within Wistar rats and large white pigs experimental models. Obtained results will be useful for retrospective dosimetry and back estimate of absorbed dose of ionizing irradiation.
RONSDOZ – Noninvasive measurement of proinflamatory markers of oxidative stress in irradiated as an indicator of received dose of radiation. Protective role of acetyl-L-carnitine
Vávrová, J., Řezáčová, M., Pejchal, J., Österreicher, J., Tichý, A.
Supported by the Czech Republic Ministry of Defence, 2008–2011 (Project No.: OVUOFVZ200806)
To evaluate whether the bioindicators of oxidative and nitrative stress and inflammations of the airways can be used as biodosimetric markers after exposure to gamma radiation, after whole-body and partial irradiation of rats. To evaluate protective effect of acetyl-L-carnitine.
Study of factors in a tissue microenviroment that influence the process of skeletal muscle reparation
Filip, S., Vávrová, J., Mokrý, J., Šinkorová, Z., Tichý, A., Čížková, D., Řezáčová, M.
Supported by the Czech Republic Grant Agency, 2008–2010 (Project No.: GA304/08/0329)
Degeneration of the cardiotoxin-damaged skeletal muscle is followed by a subsequent tissue repair, which is a multistep process that is well morphologically defined. We modify this process with gamma irradiation before and after induction of a muscle damage, which impairs a natural course of myofibre regeneration and makes a space for transplanted exogenous cells (CD117+, Sca-1+ bone marrow cells). Progeny of transplanted exogenous vector, lacZ gene, is identified histochemically. A part of the study involves examination of kinetics of haemopoietic stem cells after lethal irradiation and transplantation of stem cells.